Intracellular Ascorbate Prevents Endothelial Barrier Permeabilization by Thrombin*

The discussion of the Linus Pauling vitamin C/lysine invention for chronic scurvy

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lolex
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Intracellular Ascorbate Prevents Endothelial Barrier Permeabilization by Thrombin*

Post Number:#1  Post by lolex » Thu Oct 15, 2015 6:57 pm

FYI.... here's a recent study affirming Vitamin C's effectiveness...

promising...

http://www.jbc.org/content/290/35/21486.abstract

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OxC
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Re: Intracellular Ascorbate Prevents Endothelial Barrier Permeabilization by Thrombin*

Post Number:#2  Post by OxC » Fri Oct 16, 2015 1:31 pm

lolex wrote:FYI.... here's a recent study affirming Vitamin C's effectiveness...
http://www.jbc.org/content/290/35/21486.abstract

Thanks lolex. It is unfortunate that only the abstract is available to most people. I've read the full text, and you might be very interested to know that they used the oxidized form of vitamin C, dehydroascorbic acid, in this study:
To assess the effects of intracellular ascorbate on thrombin-induced permeability, we used DHA. DHA is the two-electron-oxidized form of ascorbate that is taken up on glucose transporters and is rapidly reduced to ascorbate inside the cells. Thus, loading cells with DHA rather than ascorbate itself avoids the potentially confounding effects of extracellular ascorbate such as redox cycling with ferric iron in the culture medium, which generates oxidative stress. After pretreating cells with DHA for 60 min, the thrombin-induced increase in endothelial permeability was blunted in a dose-dependent manner by increasing concentrations of intracellular ascorbate. Even when cells were exposed to thrombin for 30 min before addition of DHA at the beginning of the inulin transfer assay, the expected thrombin-induced increase was still reversed at even the lowest DHA concentration.

...Several conditions and diseases are characterized by both depleted ascorbate and vascular leakage, including diabetes, metabolic syndrome, inflammatory neurodegenerative diseases, and sepsis.
Douglas Q. Kitt, founder of ReCverin LLC, sellers of stabilized dehydroascorbic acid solutions.


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