Jacquie wrote:BrightSideOfLife wrote:Jacquie wrote:
120 degrees F seems far too warm to my memory of making lipsomal C at home. I believe that temperature would destroy a certain amount of your vitamin c before it gets encapsulated. If I recall correctly, I heated the mixture to 95 degrees F, or 35 C.
Liposomes will not form at 35C because the transition temperature does not occur with Lecithin below 43C. If you do not reach the transition temperature then you do not form liposomes. The only alternative is to use a different form of phosphatidylcholine with a lower transition temperature. There are many different forms of PC but most are not easily available.
45C might be higher than absolutely necessary, ~43-44C might be enough. Ascorbic acid should be okay at that temperature. 49C is higher than necessary but is around the top end of the transition range.
Your assertion contradicts the instructions on the website quoted on the first post of this thread. Do you have a source that backs up your claim for the higher temperatures being necessary?
Other things have occupied my time therefore I have not been able to reply until now. One of the problems with lecithin is that there is no standard because it contains different quantities of fatty acids which alters the transition temperature. I have come across something which seems related in that it shows temperature and ultrasonic energy applied time. I somehow doubt that you will like it because the temperature is fairly high.
Have a read and see what you think. OPTIMIZATION OF MIXING TEMPERATURE AND SONICATION DURATION IN LIPOSOME PREPARATION