Code: Select all
Baseline.
B 112
water
A 131
B 125
C 127
Gulp 250 mg Ascorbic Acid in 1 ounce water at the minute (skin prick).
Code: Select all
Meter Time Reading
A +1 162
B +2 148
C +3 140
A +4 132
B +5 146
C +6 157
A +7 158
B +8 202
C +9 137
A +10 148
B +11 157
C +12 205
A +13 247
B +14 123
C +15 160
A +16 129 * may have not rotated meters properly
B +17 147
C +18 183
A +19 130
B +20 138
C +21 139 * readjusted meters
C +22 166
A +23 159
B +24 116
C +25 138
A +26 142
B +27 269
C +28 139
A +29 124
B +30 124
C +31 167
A +32 139
B +33 116
C +34 138
A +35 218
B +36 133
C +37 180
A +38 160
B +39 122
C +40 157
A +41 184
B +42 134
Notes: Much more complex procedure. Requires at least two people, we could have used a third.
First step was measuring 250 mg of ascorbic acid using a gram scale and putting in 40 1 oz plastic cups (see picture below). (We made some extras - and we needed them - spills)
Then we began mixing as the 250 mg was hard to dissolve in 1 oz of water - required stirring.
I began the experiment by gulping one ounce (250 mg aa) at Time zero, and then pricking my finger and then gulping the next ounce every minute thereafter.
At some point I believe I missed a gulp - (maybe around minute 13 looking at the numbers) which is why we extended another minute.
I also mixed up the meter rotation at some point, but when I noticed, I corrected.
Observations.
1. Surprised that the very first minute again showed the immediate increase we have grown to expect. (only 250 mg)
2. Also different from IV in that the water was taken orally along with the vitamin C.
3. Have to wait for johnwen's graph of IV/C versus this experiment to see if any bioavailability comparison can be made.
Next experiment will likely be 10 grams of glucose - all at the same time.
Added - about 1 hour later - minor but definite bowel tolerance reaction (Something that did not happen when I gulped 10 grams ascorbic acid all at once, and according to theory, would mean that a portion of the 10 grams taken 250 mg every minute was not absorbed and made it to the rectum.)